Histology Sample Submission Hints
For best results, specimens intended for histopathological examination should be submitted in 10% formalin. Formalin must penetrate the tissue completely to preserve detail critical to accurate microscopic evaluation. For this reason, tissue must be completely immersed in a generous quantity of formalin (a tissue: formalin ratio of 1:10 is ideal). Large specimens (greater than 1 to 2 cm in diameter) should be partially incised (every 1 cm or so) so that fixative can penetrate the tissue.
For cytologies, smears of aspirates or imprints of tumors and skin lesions should be prepared immediately following collection, air-dried, and then fixed in methanol or sprayed with a commercial fixative such as Spray-cyte prior to shipment. Plastic stacking-type slide containers tend to better prevent slide breakage during transit as compared to flat cardboard or plastic slide holders. If cytology is required on a fluid, slides should be immediately prepared after the specimen is collected, and/or the fluid placed in a fixative solution (and EDTA blood tube is usually ideal) and shipped chilled overnight. A red top tube of the fluid can also be submitted chilled (and shipped overnight) for culture or other testing if desired.
Last Updated: October 9, 2008
