TRI-OLOGY

Sample Submissions

Mar/
Apr
Year to Date
Morphological Identifications 2,884 4,744
Molecular Identifications 303 602
Total Samples Submitted 3,187 5,346

Certification and Regulatory Samples

Multistate Certification for National and International Export 2,239 3,631
California Certification 437 755
Pre-movement (Citrus Nursery Certification) 34 62
Site or Pit Approval (Citrus Nursery and Other Certifications) 60 66

Other Samples

Identifications (invertebrate) 1 17
Plant Problems 33 58
Intrastate Survey, Random 80 155
Molecular Identifications* 303 602

*The majority of these analyses involved root-knot nematode species

Root galling caused by Meloidogyne javanica (Javanese root-knot nematode) on Solanum viarum (tropical soda apple)

Cyperus esculentus (yellow nut-sedge)
Photograph and copyright courtesy of Shirley Denton, Atlas of Florida Vascular Plants

Melaleuca viminalis (bottle brush)

Meloidoygne mayaguensis Rammah and Hirshmann, 1988 (the guava root-knot nematode) infecting the roots of Melaleuca viminalis (bottle brush)
Photograph courtesy of Mariana P. Beckman and Janete A. Brito, DPI

Nematology Section

Compiled by Janete A. Brito, Ph.D., and Renato N. Inserra, Ph.D.

This section analyzes soil and plant samples for nematodes, conducts pest detection surveys and provides diagnosis of plant problems, in addition to completing identification of plant parasitic nematodes involved in regulatory and certification programs. State of Florida statutes and rules mandate the principal part of the regulatory activity of the section. Analyses of plant and soil samples include those from in-state programs, plant shipments originating in Florida destined for other states and countries, as well as samples intercepted in Florida from outside the United States.

Nematodes of Special Interest

Heterodera cyperi Golden, Rau & Coob, 1962 (a cyst-forming nematode) was infecting the roots of Cyperus esculentus (yellow nut-sedge) growing in a potato field. Although this species has been detected in Florida since early 1960s and is widely distributed in cultivated fields and natural land, H. cyperi population levels are low and do not cause appreciable damage to yellow nut-sedge. It seems that many biological control agents (especially fungi) prevent the nematode from reaching high populations levels. The mild parasitic relationship between the nematode and the weed allows the nematode to develop and to reproduce on the weed roots without affecting the vigor of the host plant. (St. Johns County, N09-00420, Trevor R. Smith, 27 March 2009.)

Meloidoygne mayaguensis Rammah and Hirshmann, 1988 (the guava root-knot nematode) was found infecting the root of Melaleuca viminalis (bottle brush). The root system was heavily galled with visible egg masses on the surface of the roots.  This nematode species has been found infecting several other horticultural crops and weed plants in the state. New county record. (Clay County, N09-00508, Sol F. Looker, 9 April 2009). (Brito, et al. 2008.)

References

Collectors submitting five or more samples that were processed for nematological analysis in March - April 2009

Anderson, James L. 192
Bentley, Michael A. 46
Bloom, Richard T. 5
Burgos, Frank A. 180
Edenfield, Carrie S. 63
LeBoutillier, Karen W. 205
Ochoa, Ana L. 186
Pate, Jo Ann 59
Qiao, Ping 160
Robinson, William L. 8
Spriggs, Charles L. 299
Toral, Angelina M. 10