TRI-OLOGY

Sample Submissions

Jan/
Feb		 Year to Date
Morphological Identifications 2,193 2,193
Molecular Identifications 113 113
Total Samples Submitted 2,306 2,306

Certification and Regulatory Samples

Multistate Certification for National and International Export 1,466 1,466
California Certification 539 539
Pre-movement (Citrus Nursery Certification) 56 56
Site or Pit Approval (Citrus Nursery and Other Certifications) 17 17

Other Samples

Identifications (invertebrate) 1 1
Plant Problems 16 16
Intrastate Survey, Random 98 98
Molecular Identifications* 113 113

*The majority of these analyses involved root-knot nematode species

Opuntia humifusa infected with Meloidogyne mayaguensis
Opuntia humifusa infected with Meloidogyne mayaguensis
Opuntia humifusa infected with Meloidogyne mayaguensis

Opuntia humifusa infected with Meloidogyne mayaguensis. Note the root galling induced by this nematode species.
Photograph courtesy of Darlene J. George-Hill, DPI.

Nematology Section

Compiled by Janete A. Brito, Ph.D. and Jason D. Stanley, M.S.

This section analyzes soil and plant samples for nematodes, conducts pest detection surveys and provides diagnosis of plant problems, in addition to completing identification of plant parasitic nematodes involved in regulatory and certification programs. State of Florida statutes and rules mandate the principal part of the regulatory activity of the section. Analyses of plant and soil samples include those from in-state programs, plant shipments originating in Florida destined for other states and countries, as well as samples intercepted in Florida from outside the United States.

Nematodes of Special Interest

Nematodes of special interest detected and/or identified in January – February 2010

Meloidogyne mayaguensis infects agronomic and horticultural crops as well as natives and weed plants. Infected plants exhibit root galling, which is the major symptom induced by this nematode species. This nematode species has been reported in Africa, Asia, South America, Central America, North America and the Caribbean region. In North America, it is known to occur only in Florida. This root-knot nematode reproduces on 13 non-agronomic species worldwide. Certain weeds species are of particular importance because they can serve as a major reservoir of root-knot nematodes not only in the field, but also in nurseries, and should be included in nematode management programs.

Meloidogyne mayaguensis Rammah & Hirschmann, 1988, (the Guava root-knot nematode) a new Host record, was found infecting the root system of a wild-growing plant, Opuntia humifusa (prickly pear cactus). (Miami-Dade County, N10-00103, Ana L. Ochoa, 26 January 2010.)

Meloidogyne mayaguensis Rammah & Hirschmann, 1988, (the Guava root-knot nematode) a new Host record, was found infecting the root system of an ornamental plant, Lagerstroemia indica (crape myrtle). (Lake County, N10-00089, Charles L. Spriggs, 11 November 2009.)

Meloidogyne mayaguensis Rammah & Hirschmann, 1988, (the Guava root-knot nematode) a new Host record, was found infecting the root system of a weed, Paederia cruddasiana (sewer vine). (Miami-Dade County, N09-01142, Ana L. Ochoa, 4 September 2009.)

Collectors submitting five or more samples that were processed for nematological analysis in January - February 2010

Anderson, James L. 94
Bailey, Wayne W. 22
Bentley, Michael A. 21
Burgos, Frank A. 216
Edenfield, Carrie S. 65
LeBoutillier, Karen W. 105
Ochoa, Ana L. 191
Pate, Jo Ann 57
Qiao, Ping 164
Smith, Larry W. 11
Spriggs, Charles L. 119
Tannehill, Ellen J. 6

References

Brito, J. A., R. Kaur, R. Cetintas, J. D. Stanley, M. L. Mendes, E. J. McAvoy, T. O. Powers and D. W. Dickson. 2008. Identification and isozyme characterization of Meloidogyne spp. infecting horticultural and agronomic crops and weed plants in Florida. Nematology 10:757-766.

Rich, J. R., J. A. Brito, R. Kaur and J.A. Ferrell. 2009. Weed species as hosts of Meloidogyne: a review. Nematropica 39: 157-185. Retrieved March 10, 2010, from http://www.ontaweb.org/.